Sporosarcina ureae ATCC

The S-layer protein of Sporosarcina ureae ATCC 13881, observed for the first time in 1979, has not yet been fully characterized with respect to its selfassembling ability. Genetic and structural information exists, however in comparison to other S-layers, there is a lack of knowledge concerning the self-assembling structures it forms under different in vitro recrystallization conditions. Moreover, up to now, there are no data available on which part of the protein is responsible for its remarkable self-assembling property. 

Sporosarcina ureae ATCC 13881 is a Gram-positive, motile bacterium living in soil. Taxonomically, it belongs to the family of Bacillaceae under the genus Sporosarcina. It requires alkaline conditions for growth (pH 8.8 is optimal) and is able to form endo-spores (Goldman and Wilson, 1977 MacDonald and MacDonald, 1962). 

The native S-layer of Sporosarcina ureae ATCC 13881 was recrystallized at (a) low protein concentration (0.29 irg/jj, ) over a time period of 1 month, (b, c) higher protein concentration (0.79 [j,g/ij,l) over a time period of 12 days. 

Ryzhkov, P., 2007. Bioengineering of S-layers molecular characterization of the novel S-layer gene SslA of Sporosarcina ureae ATCC 13881 and nanotechnology application of SslA protein derivatives (Dissertation). TU Dresden. 

Ryzhkov, P., Ostermann, K., Rodel, G., 2007. Isolation, gene structure and comparative analysis of the S-layer gene sslA of Sporosarcina ureae ATCC 13881. Genetica 131, 255-265. 

---

See also in sourсe #XX -- [ , , , ]

---