Spinach carotenoid pigments isolation

Isolation of Chlorophyll and Carotenoid Pigments from Spinach... 

Not only/3-carotene but alsooC -carotene, lutein, violaxanthin, neoxanthin and presumably other carotenoids may form LVM s. It seems likely that the LWAFs of pigments are formed mainly under appropriate conditions in artificial systems. However, the possibility cannot be excluded that under suitable conditions they may also be encountered in chloroplasts. For example, when using DEAEcc we were able to isolate the /3-carotene LWAF only from winter spinach (Wipckowski et al., 1981). So it seems likely that it occurred in the chloroplasts prior to treatment with Triton X-100. 

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