Sphingolipid Metabolites

Coroneos, E., Wang, Y., Panuska, J.R., Temepleton, D.J. and Kester, M., 1996, Sphingolipid metabolites differentially regulate extracellular signal-regulated kinase and stress-activated protein kinase cascades, Biochem. J. 316 13-17. 

Mano, N., Oda, Y. and Yamada, K. (1997) Simultaneous quantitative determination method for sphingolipid metabolites by liquid chromatography/ionspray ionization tandem mass spectrometry. Anal. Biochem., 244 (2), 291-300. 

Figure 10 shows interrelationships among the sphingolipid metabolites. Ceramide is converted to glycosylceramides and more complex glycosphingolipids and to 1-0-acylceramide... 

Irradiahon (UV and y rays), chemotherapy, pathogenic infec-hons, and many other external stress stimuli activate SMases and elevate the endogenous levels of ceramide in tumor cells, which promotes apoptosis. However, tumor cells may counteract these treatments by activation of ATP-dependent efflux proteins and by converting endogenous ceramide to other sphingolipid metabolites, which thereby evades apoptosis. 

Ceramide and sphingosine-1-phosphate are formed as a result of the hydrolysis of membrane sphingolipids. Recent work has often emphasized the antiproliferative aixl differentiation-inducing effects of ceramides and sphingolipid metabolites have been... 

Wang, F, Buckley, NE, Ohvera, A, Goodemote, KA, Su, Y and Spiegel, S (1996) Involvement of sphingolipids metabolites in cellular proliferation modulated by ganglioside GMl. Glycoconj J, 13, 937-945. 

Figure 8.4 Representative product-ion ESI-MS spectra of sphingolipid metabolites in the positive-ion mode after low-energy CID. Product-ion ESI-MS analyses of protonated sphin-gosine (a), SIP (b), lysoSM (c), and psychosine (d) were performed at collision energy of 15, 24, 22, and 24 eV, respectively, by using a QqQ mass spectrometer (Thermo Fisher TSQ Vantage). Gas pressure of 1 mTorr was employed in the collisional activation.

Overall the dishnctive physicochemical properties of bioachve sphingolipid metabolites accormt for various regulatory effects on a broad range of brain cells, including neurons, oligodendrocytes, and astrocytes." ... 

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