Spex 6750 freezer mill

Root powdering and demineralization. Sixty-five frozen and pulp-free tooth roots were ground in a Waring blender and powdered under liquid nitrogen in a freezer mill (Spex, Edison NJ, USA). The sieved powder (< 450 pm) was demineralized in dialysis bags in 0.5 M EDTA, pH 7.4, at 4°C with regular replacement of the solution. Demineralization was continued until no further calcium release could be detected by atomic absorption spectrometry. 

Freezer mill SPEX CertiPrep (Metuchen, NJ) Y N Y N Y Yu and Cohen, 2003... 

M SPEX Certiprep Mixer Mill, 2.0x0.5 in. stainless steel vial, 0.150 in. Pall. t SPEX Certiprep freezer mill, -196°C, 30min aldehyde NaH 10 1 mol. 

Liquid nitrogen pulverizer (Freezer-Mill, Spex)... 

Seven of the artifacts from the La Scola site were analyzed by ICP-MS. Samples were ultrasonically cleaned and dried, and then pulverized at liquid nitrogen temperatures using a SPEX 6700 freezer mill. 100 mg of the powdered sample were mixed with 0.2 mL aqua regia and 2.5 mL hydrofluoric acid and dissolved in a Parr acid digestion bomb at 120 °C for 2 hours. Samples were then diluted to a total volume of250 ml so that elements of interest would be present at concentrations of 500 ppb or less. 

The PP pellets were ground into a powder using a SPEX CertiPrep Freezer Mill (Metuchen, NJ). The pellets were precooled for 5 min followed by three 2min grinding cycles at lOHz. Between each cycle the sample was cooled for a Imin interval. Nanocomposites were created by dry blending the PP powder with a given ratio of MWNTs (0.25, 0.50, 1 and 3 wt%). 

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