Spectroscopy photosynthetic reaction centers

Thompson MA, Zemer MC (1991) A theoretical examination of the electronic structure and spectroscopy of the photosynthetic reaction center from Rhodopseudomonas viridis. J Am Chem Soc 113 8210-8215... 

Ultrafast Dynamics and Spectroscopy of Bacterial Photosynthetic Reaction Centers 1... 

The next two chapters are devoted to ultrafast radiationless transitions. In Chapter 5, the generalized linear response theory is used to treat the non-equilibrium dynamics of molecular systems. This method, based on the density matrix method, can also be used to calculate the transient spectroscopic signals that are often monitored experimentally. As an application of the method, the authors present the study of the interfadal photo-induced electron transfer in dye-sensitized solar cell as observed by transient absorption spectroscopy. Chapter 6 uses the density matrix method to discuss important processes that occur in the bacterial photosynthetic reaction center, which has congested electronic structure within 200-1500cm 1 and weak interactions between these electronic states. Therefore, this biological system is an ideal system to examine theoretical models (memory effect, coherence effect, vibrational relaxation, etc.) and techniques (generalized linear response theory, Forster-Dexter theory, Marcus theory, internal conversion theory, etc.) for treating ultrafast radiationless transition phenomena. 

Rigby, S.E.J., Evans, M.C.W., and Heathcote, P. (2000) Electron nuclear double resonance (ENDOR) spectroscopy of radicals in photosystem I and related type 1 photosynthetic reaction centers, Biochim. Biophys. Acta 1507 247-259. 

Middendorf, T. R., Mazzola, L. T., Lao, K., Steffen, M. A., and Boxer, S. G., 1993, Stark effect (electroabsorption) spectroscopy of photosynthetic reaction centers at 1.5 K Evidence that the special pair has a large excited-state polarizability. Biochim. Biophys. Acta, 1143 223fi234. 

Zinth, W., and Kaiser, W., 1993, Time-resolved spectroscopy of the primary electron transfer in reaction centers of Rhodobacter sphaeroides and Rhodopseudomonas viridis. In The Photosynthetic Reaction Center, (J. Deisenhofer and J. R. Norris, eds.) Volume 2, 71988, Academic Press, San Diego, USA. 

Fig. 9. (A) Absorption spectrum of Rb. sphaeroides used as a reference to show the Qx and Qy bands of the primary donor (P), BChl [B] and bacteriopheophytin [BO] (B) Femtosecond absorption changes at 920 (a), 785 (b) and 545 nm (c) vs. the delay time of the monitoring pulse measured at room temperature, and (C) absorption changes at 920 (a) and 794 nm (b) measured at 25 K. Figure source (A) see Fig. 7 (B) Holzapfel, Finkele, Kaiser, Oesterheldt, Scheer, Stilz and Zinth (1990) Initial electron transferin the reaction center from Rhodobacter sphaeroides. Proc Nat Acad Sci, USA 87 5170 (C) Zinth and Kaiser (1993) Time-resolved spectroscopy of the primary electron transfer in reaction centers of Rhodobacter sphaeroides and Rhodopseudomonas viridis. I n JR Norris and J Deisenhofer (eds) The Photosynthetic Reaction Center, Voi il, p 82. Acad Press.

In this review, we explain the SAC-CI applications to molecular spectroscopy with some examples. In Section 2, we briefly explain the theoretical and computational aspects of the SAC-CI method. Then, we show some SAC-CI applications to molecular spectroscopy the excitation and ionization spectra of tt-conjugated organic molecules (Section 3), collision-induced absorption spectra of van der Waals complex (Section 4), excitation spectra and NMR chemical shifts of transition metal complexes (Section 5), photofragmentation reaction of Ni(CO)4 (Section 6), absorption spectrum of free-base phthalocyanine (FBPc) and bacterial photosynthetic reaction center... 

Zhou Q, Robert B and Lutz M (1987) Intergeneric stmctural variability of the primary donor of photosynthetic bacteria Resonance Raman spectroscopy of reaction centers from two Rhodospirillum and Rhodobacter species. Biochim Biophys Acta 890 368-376... 

Carotenoids function in photosynthetic reaction centers (RC) as triplet quenchers of the primary donor chlorophyll or bacteriochlorophyll triplet states. The best studied RCs are those of purple photosynthetic bacteria where atomic models are available based on X-ray crystallography and optical as well as magnetic resonance spectroscopies have yielded a detailed picture of the flow of triplet energy transfer. Good reviews of these topics can be found in (Frank, 1992, 1993 Frank and Cogdell, 1996). 

Frank HA (1992) Electron paramagnetic resonance studies of carotenoids. Meth Enzymol 213 305-312 Erank HA (1993) Carotenoids in photosynthetic bacterial reaction centers Structure, spectroscopy, and photochemistry. In Deisenhofer J andNorris JR(eds) The Photosynthetic Reaction Center, Vol II, pp 221-237. Academic Press, San Diego Prank HA and CogdeU RJ (1996) Carotenoids in Photosynthesis. Photochem Photobiol 63 257-264... 

Kleima FJ, Gradinaru CC, Calkoen F, Van Stokkum IHM, Van Grondelle Rand Van Amerongen H (1997) Energy transfer in LHCH monomers at 77K studied by sub-picosecond transient absorption spectroscopy. Biochemistry 36 15262-15268 Kleinherenbrink FAM, Hastings G, Wittmershaus BP and Blankenship RE (1994) Delayed fluorescence from Fe-S type photosynthetic reaction centers at low redox potential. Biochemistry 33 3096-3105... 

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