Spectromicroscopy, synchrotron-based

The analysis of MDA-MB-231 cells incubated with 53 was also carried out on a synchrotron-based multiple beam FT-IR imaging (IRENI) set up at the Synchrotron Radiation Center, Stroughton, Wisconsin, equipped with a FPA detector of 96 X 96 pixels, where each pixel corresponds to a 0.54 x 0.54 pm area of the sample. With the better resolution afforded by this technique, the complex appears heterogeneously distributed in the cell, but with a higher perinuclear concentration [79]. In addition, 53 was mapped in cell by SR-UV-SM (synchrotron radiation UV spectromicroscopy), confocal Raman microscopy, and AFM-IR (see Sections 11.3.3 and 11.3.4). Thus, this innovative family of complexes appeared to be a valuable multimodal (and not only bimodal) tool for cell imaging. 

H. Y. N. Holman, M. C. Martin and W. R. McKinney, Synchrotron-based FTIR spectromicroscopy Cytotoxicity and heating considerations, /. Biomed. Phys., 2003, 29, 275-286. 

Synchrotron-Based Infrared Raster-Scanned (IR SR) Spectromicroscopy... 

Table 15.1 Spectral noise comparison between synchrotron-based raster scanning and widefieid spectromicroscopy.

---