Spectrometric Identification of Proteinous Allergens

It has been of interest to identily and characterize the molecules responsible for type I allergy to elderberry. ID gel electrophoresis of elderberry flowers, pollen and berry extracts showed a very complex protein composition (Fig. 2). Under nonreducing conditions clearly two dominant gel bands at 33 and 66 kDa were visible after Coomassie staining, which coincide at 33 kDa under reducing circumstances 

2D gel electrophoresis, carried out to further characterize the physicochemical properties of the allergen complex, clearly exhibited five spots (labeled a-e in Fig. 3 A) 

Summary of the 12 peptides and their amino acid sequences revealed after MS/MS experiments obtained either on a MALDI-QfT/RTOF or on an off-hne nano-ESl-QIT mass spectrometer 

Peptide [M + H]+ Amino acid sequence determined by MALDl-QIT/RTOF-MS Amino acid sequence determined by ESI-Qfr-MS Combined sequence information used for BLAST Relevant BLAST result 

Complementing results of the instruments made the elucidation of long stretches feasible resulting in seven positive BLAST results relevant for Sambucus species. 

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