Specificity chimeric nuclease

Fig. 6.3 Molecular model of the domains of the chimeric nuclease (constituted by an hybrid between a non-specific DNA cleavage domain and a zinc finger recognition domain) and DNA. The cleavage domain sits behind...

Semiartificial nucleases include (1) in concept, chimeric nucleases which are composed of heterogenous structural parts brought together by the use of recombinant DNA techniques and (2) hybrid nucleases which are derived from classical nucleases by incorporating a piece of synthetic oligonucleotide in the active site to harness a sequence specificity. An example of the hybrid nuclease is described in Section II (this chapter) on staphylococcal nuclease. 

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