Specific Considerations for Analysis of Enzymes Using XPS

1 Specific Considerations for Analysis of Enzymes Using XPS To fully 

FIGURE 13.8 Schematic showing principle of angle-resolved XPS. 

Furthermore, no extensive reference library exists for enzymatic materials. It is critical, therefore, to design an experiment such that individual constituents of the biocomposite, such as dry enzymes, solvent-cast enzymes, substrates, and polymers, are aU analyzed to provide appropriate reference spectra. The respective widths and positions of peaks in the reference spectra can then be applied as constraints in curve fitting the spectra of the eomposite samples (enzyme encapsulated in matrix, layer-by-layer systems, etc.). Identification of species must be then cross-correlated and confirmed by spectra of all the elements that take part in the suspected type of chemical bond formation— for example, peaks due to C—N=0 must be confirmed by C Is, O Is, and N Is signals. 

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