Spawn Formulas

When these calcium buffers are used, add 10% more water than that listed above. 

Once the grain filled jars have been autoclaved, they should be placed in the sterile room and allowed to cool. Prior to this point, the room and its air should be disinfected, either through the use of traditional cleaning methods, HEPA filters or both. 

Upon removing the warm jars from the pressure cooker or autoclave, shake them to loosen the and to evenly distribute wet and dry kernels. Shaking also prevents the kernels at the bottom the jar from clumping. 

An excellent tool to help in this procedure is a bald car tire or padded chair. Having been care-jfully cleaned and disinfected, the tire should be mounted in an upright and stable position. The tire. has a perfect surface against which to shake the jars, minimizing discomfort to the hands and reduc- 

When the grain jars have returned to room temperature, agar to grain inoculations can com- 

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MILLET Although having a higher number of inoculation points than rye, it is more difficult to formulate as spawn. Amycel, a commercial spawn-making company, has successfully developed a formula and process utilizing millet as their primary spawn medium. 

The optimum moisture content for grain in the production of spawn is between 49-54%, The following formulas are based on cereal rye grain, Secale cereale, which usually has a moisture content of 11%. Some variation should be expected depending on the brand, kernel size, geographical origin and the way the grain has been stored. 

To produce grain spawn of 48-52% moisture use the formulas outlined below and autoclave in a pressure cooker for 1 hour at 15-1 8 psi. Note that considerable variation exists between measuring cups, differing as much as 10% in their volumes. Check the measuring cup with a graduated cylinder. Once standardized, fashion a "grain scoop" and a "water scoop" from a plastic container to the proportions specified below. 

The ab initio multiple spawning (AIMS) method is a time-dependent formula-ti(Mi of quantum chemistiy which has been developed to carry out first principles... 

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