Somatostatin-like material

Acid ethanol, 10 vol, (0.2 N HC1/75 ethanol) was used to extract the somatostatin-like material from the homogenized cells. The suspension was mixed overnight at 4 °C followed by centrifugation. The ethanol was evaporated and the remaining supernatant was neutralized with NHijOH and boiled. The mixture was centrifuged and the supernatant was applied to a Sep-Pak C- q disposable cartridge (8) equilibrated in 0.1 TFA. The acetonitrile/TFA was removed by lyophilization and each fraction was assayed for somatostatin-like immunoactivity. The fractions with somatostatin activity were pooled, and purified on HPLC and tested in a bioassay (14). 

Following the partial purification of the acid-ethanol extract on Sep-Pak C ig cartridges, the eluate fractions containing somatostatin immunoactivity were pooled, lyophilized and further purified on HPLC (Figure 6). The somatostatin-like material from... 

Figure 6. Somatostatin-like material in Tetrahymena pyriformis. Key (right panel) , synthetic somatostatin O, Tetrahymena extract (HPLC purified) Band , anti-somatostatin antibody added. (Reproduced from Ref. 8.)...

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