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Solvent strength optimization gradient elution

Similarly, in gradient elution HP-RPC, resolution optimization can take advantage of the relationship between the gradient retention time of an analyte (expressed as the median retention factor k) and the median volume fraction of the organic solvent modifier, Ip, in regular HP-RPC systems based on the concepts of the linear solvent strength theory,1 31 such that... [Pg.16]

The composition of mixed mobile phases for ternary or quaternary isoselective gradient elution can be optimized using overlapping resolution mapping strategy to adjust optimum separation selectivity based on seven or more initial experiments with solvent mixtures of approximately equal elution strengths. Based on the retention data from the initial experiments, either 3-D diagrams or contour resolution... [Pg.1044]


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See also in sourсe #XX -- [ Pg.476 ]




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Elution optimization

Elution solvent

Gradient elution

Gradient strength

Gradients optimization

Optimization gradient elution

Optimization strength

Solvent gradient elution

Solvent optimization

Solvent strength

Solvent strength optimization

Solvents eluting

Solvents gradients

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