Solvent gradient interaction chromatography

SGIC2D Two-dimensional solvent gradient interaction chromatography... 

Solvent gradient interaction chromatography (SGIC) can be used to analyze copolymers in the whole range of 0-100% of comonomer incorporation, which was not possible with crystaUizatiOTi techniques. 

The most recent development in separation is the development of high temperature interaction chromatography, which extends the composition distribution analysis to polyolefin copolymers of very low crystallinity, which is not possible to analyze by crystallization techniques. The analysis of complex polymers with different composition can be analyzed in a short time by solvent gradient interaction chromatography, SGIC, on an atomically flat surface like carbon or molybdenum sulfide packing. The addition of a second separation step by GPC (SGIC2D) provides the capability to obtain full composition-molar mass dependence. 

Hydrophilic interaction chromatography (HELIC) was performed on Polyhydroxy-ethyl Aspartamide (PH ), obtained from PolyLC, packed in a PEEK column (0.53 mm ID x 3 cm) bounded by a stainless steel frit at the inlet and a glass fiber (Whatman GF/F) plug held in place by a mounted fused silica transfer line. Solvent A was 0.1% TFA solvent B was 0.1% TFA, 90% acetonitrile. Peptide solutions were applied to the column after they were brought to 90% acetonitrile. The column was equilibrated in 100% B to a stable 214 nm absorbance after injecting the sample and reestablishing the stable absorbance, a gradient of 100% to 0% B was applied to the column over 20 minutes, followed by 0% B for another 10 minutes. 

Hydrophilic interaction chromatography (HILIC) is a variation of normal-phase chromatography in which solutes are retained on a polar bonded phase under high concentrations (80-90%) of organic solvent and released during a gradient to a more aqueous solvent. The organic mobile phase usually causes at least partial denaturation of proteins. 

Because of the different separation mechanisms employed in interaction chromatography, the solvents used are usually different from the solvents in SEC. Binary or ternary mixtures of organic and aqueous solvents are frequently used as the mobile phase. The ratio of the solvents may be constant during the chromatographic run (isocratic elution) or may be programmed to vary with time (gradient elution). 

NP hydrophilic interaction chromatography gradients—concentration(s) of water (or of water and a more polar organic solvent) in a less polar solvent increase(s) aqueous mobile phases, polar adsorbent, or bonded-phase columns... 

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