Solution-based macromolecules modes

The appearance of anomalous HO elution patterns has been interpreted In two wqys (ref. 48). The first explanation is based on computer simulations of HD elution profiles of ligand-mediated association-dissociation equilibria of the macromolecule (ref. 41). The initially narrow band of N departs from the top of the chromatographic column and starts to equilibrate with the solution of L to form complexes ML, M2L etc. just as in the normal HD runs. Simultaneously, the concentration of M and its complexes decrease continuously as their band spreads, and these complexes will dissociate. Typically, a trailing elution profile of the ost peak is formed. The exact peak shape and size depends on the mode and strength of associations and on the concentration of components (ref. 41). The accordance of the theoretical predictions with actual experimental model systems has not been tested. 

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