Solubility, ADCs

One further step toward calibration has been taken with the use of a calibration curve made with sets of beads with known numbers of fluorochromes on their surface. Such calibrated beads are available with known numbers of PE molecules. Similar, but less direct, beads are available with fluorochrome molecules that have been calibrated in units equivalent to the intensity of fluorochrome molecules in solution ( MESF units = molecular equivalents of soluble fluorochrome ). With these beads, a curve can be obtained (Fig. 6.6), giving each channel on the ADC a calibration in number of fluorochrome molecules (for PE) or MESF values (for fluorescein). In this way, the background fluorescence of a control sample can be expressed as an equivalent number of fluorochrome (or MESF) molecules and can be subtracted from the number of fluorochrome molecules of a stained sample. The fluorescence of the stained sample can then be expressed as, for example, PE molecules over and above the background level. 

The rate of photosynthesis was measured using a portable IRGA (ADC, England). Chloroplast isolation and measurements of photochemical activity which included PSII, PSI and whole chain electron transport activities, were carried out according to the procedure described by Sayeed and Mohanty (7). Estimation of chlorophyll was carried out following Arnon (8) and soluble leaf proteins were estimated following Lowry et al (9). 

Fukukawa et al. prepared semialicyclic fluorinated polyhydroxyamide (PAHA) containing adamantyl units by the reaction of 1,3-adamantanedicarbonyl chloride (ADC) and 4,4 -(hexafluoroisopropylidene) bis(c-aminophenol) (6FAP). Corresponding PBOs (Figure 5.35) were obtained by thermal treatment of the PAHA. The precursor polymers were soluble in... 

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