Solid support, sequence-specific

A possibility to overcome the time-consuming solution-phase peptide synthesis, but avoiding specific synthetic problems that cannot be addressed on tbe solid support, is to follow a combined solution-/solid-pbase approach. In such an approach the majority of the peptide sequence is typically assembled on solid support, but critical steps are performed in solution. Below, some exemplary peptides are discussed. 

In situ hybridization was derived from the techniques of molecular hybridization of nucleic acids that are isolated from a particular cell population or tissue and bound to solid supports. Hybridization of such averaged membrane-bound nucleic acids identifies different classes of DNA (Southern blot) and RNA (Northern blot). However, ISH fills the gap between the detection of a specific sequence and its precise location within the tissue or the cell (Chevalier et al., 1997). 

Young, J. K. Nelson, J. C. Moore, J. S. Synthesis of Sequence Specific Phenylacetylene Oligomers on an Insoluble Solid Support, J. Am. Chem. Soc. 1994,776, 10841. 

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