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Solid phase synthesis of cysteine-containing peptides

2 Solid-phase synthesis of cysteine-containing peptides [Pg.87]

Protocol 3. Esterification of Fmoc-Cys(Trt)-OH onto 2-chlorotrityl chloride resin (49,53) [Pg.87]

Wash the 2-chlorotrityl chloride resin (typical loading 1.0 mmol/g) with CHjCtj. [Pg.87]

Add a solution of Fmoc-Cys(Trt)-OH 3 eq with respect to Cl groups on resin) and DIPEA (3.3 eq) in a minimal volume of CH2CI2. [Pg.88]

Terminate the reaction by adding MeOH (1 pl/1 pmol of starting chloride). [Pg.88]




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Peptide solid phase

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Solid-phase synthesi

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