Solid phase organic synthesis peptide arrays

Out of the combinatorial chemistry boom came the framework for modem solid-phase organic synthesis. While a lot of the early work with SPOS focused on reliable and relatively straightforward peptide coupling reactions, the ambitious library syntheses of the 1990s required access to a much more extensive array of solid-phase reactions. That decade saw initial strides made in adapting many well-known solution-phase reactions for use in the solid-phase arena, development that continues to the present day, " and a move beyond peptide and nucleotide chemistry toward preparation of small molecule libraries on solid phase. 

Positional scanning, or 1-D indexing, was reported as a deconvolutive technique by Houghten and coworkers [35] and subsequently applied extensively to solid-phase peptide library synthesis. An example of solid-phase small organic molecule 2-D indexed library synthesis and deconvolution was reported by Berk et al. [36]. Description of this strategy, called spatially arrayed mixture (SpAM), and a comparison with classical positional scanning, are shown in Figure 8.9. 

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