Solid phase extraction elution pattern

Figure 4 Elution patterns of hyoscine (scopolamine) and its dehydrated metabolite, apohyoscine, from Cis SPE columns. Hyoscine (A) or apohyoscine (B) eluted with 40% (v/v) methanoliwater and hyoscine (C) or apohyoscine (D) eluted with 50% (v/v) metha-noliwater. The latter eluent was chosen for the assay as >90% of hyoscine was recovered in the first milliliter, whereas <5% of the apohyoscine was eluted in this volume. Washing with 20% (v/v) methanol removed the majority of the urinary pigments but not the hyoscine. (Reproduced with permission from Whelpton R, Hurst PR, Metcalfe RF, and Saunders SA (1992) Liquid chromatographic determination of hyoscine (scopolamine) in urine using solid phase extraction Biomedical Chromatography 6. 198-204 John Wiley Sons Ltd.)...

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