Sodium periodate antibodies

Dissolve sodium periodate in water to a final concentration of 100 mM. Protect from light. Add 0.1 ml of this stock periodate solution to each ml of the antibody solution. 

Figure 10.3 Antibody molecules oxidized with sodium periodate to create aldehyde groups on their polysaccharide chains can be modified with PDPH to produce thiols after reduction of the pyridyl disulfide. Direct labeling of the sulfhydryls with "Tc produces a radioactive complex.

Figure 20.8 Enzymes that are glycoproteins like HRP may be oxidized with sodium periodate to produce reactive aldehyde residues. Conjugation with an antibody then may be done by reductive animation using sodium cyanoborohydride.

A major achievement in augmenting the chemical potential of antibodies has been in the area of redox processes. Many examples now exist of stereoselective reductions, particularly recruiting sodium cyanoborohydride (Appendix Section 22). A growing number of oxidation reactions can now be catalysed by abzymes, with augmentation from oxidants such as hydrogen peroxide and sodium periodate (Appendix Section 21). 

Modifications are made also in order to use this reaction to conjugate nucleosides to erythrocyte surfaces, allowing use of the coated cells as targets for assays of antibody-forming splenic lymphocytes. Nucleoside, 10-20 mg, is oxidized in 1.5 ml of 0.1 M sodium periodate in 0.15M NaHCOa for 20 min at room temperature the reaction is stopped by the addition of 15 /zl of ethylene glycol. Sheep erythrocytes are washed twice with 0.15 M NaHCOs, and 0.5 ml of packed cells is then suspended in 2.0 ml of the bicarbonate solution in a40-ml centrifuge tube. The oxidized nucleoside is added dropwise to the cell suspension and the mixture is kept at room temperature for 15 min. fert-Butylamine borane (Aldrich Chemical Co.), 100 mg in 5 ml of 0.15 M NaHCOs, is added. The suspension is kept at room temperature for 3 min, and the tube is then quickly filled with bicarbonate solution and centrifuged at 1500 rpm for 10 min. 

Immediately add the antibody solution to the sodium periodate solution and stir at room temperature for 1 h. 

Directed IgG immobilization on gold by covalently linking sodium periodate-oxidized antibody to polylysine and linking the IgG-polylysine complex to alkanethiols on the gold surface Antigen [8]... 

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