Sodium cyanoborohydride handling

In a fume hood, dissolve 125 mg of sodium cyanoborohydride in 1ml water (makes a 2M solution). Caution Highly toxic compound handle with care. This solution may be allowed to sit for 30 minutes to eliminate most of the hydrogen-bubble evolution that could affect the vesicle suspension. 

In a fume hood, add 0.2 ml of 1 M sodium cyanoborohydride (Aldrich) to each ml of the protein/dextran solution. Mix well. Caution Cyanoborohydride is extremely toxic and should be handled only in well-ventilated fume hoods. Dispose of cyanide-containing solutions according to approved guidelines. 

Incubate the reaction mixture for 120 min at 4°C. Sodium cyanoborohydride is extremely toxic and should only be handled in a fume hood while wearing appropriate personal protective equipment. Cyanoborohydride-containing solutions also contain cyanide and must be disposed of according to appropriate local regulations. 

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