Size exclusion chromatography spectra

Figure 5. Size exclusion chromatography spectra. Key +, Table, experiment 2,b. O, Table, experiment Lb. A, Table, experiment 2.a. Table, experiment l.a.

Figure 15.1. MALDI spectrum of a polycarbonate sample along with peak assignment. In the inset, an expansion of the spectral region from 3.0 up to 3.7 kDa is shown. (Reproduced from Puglisi, C. et al., 1999. Analysis of Poly(bisphenol A Carbonate) by Size Exclusion Chromatography/Matrix-Assisted Laser Desorption/lonization. I. End Group and Molar Mass Determination. Rapid Communications in Mass Spectrometry, 13 2260-2267. With permission of John Wiley Sons, Inc.)...

A complementary use of polymer viscometry is the indirect evaluation of the MWD of a polymer from dynamic viscosity measurements [28-30]. The methods used to correlate the MWD of polymers to rheological data are based on the previous determination of the polymer relaxation spectrum from linear oscillatory shear experiments [31, 32]. MWDs obtained from viscometric data analysis can help in the determination of the MWD curve from online measurements, or in cases where this curve cannot be easily determined from size exclusion chromatography (SEC) [30, 31]. 

Figure 1. Purification of rat CpnIO (101 residues) using Fmoc probe 2. (A) Analytical RP-HPLC (C4 medium) of crude underivatized rat CpnIO. (B) Addition of lipophilic probe 2 increases the retention time of the protein (labelled 2) thus facilitating purification from underivatized truncated sequences (labelled 1). (C) Purified protein derivatized with 2. (D Purified protein after treatment with 5% aqueous TEA to remove 2. (E) ESI-MS of purified rat CpnIO. (R Deconvoluted mass spectrum for purified rat CpnIO. The calculated mass of target product is 10770.57 Da (average). The found mass is 10771.0. (Q) RP-HPLC (C4 medium, gradient TFA-water into 100% TFA-AcCN, 60 min) of purified rat CpnIO. The insert shows the expanded peak. (H) CZE of purified rat CpnIO. The concentration of the major peak (protein in its native, heptameric state) is 84%. Separate size-exclusion chromatography experiments showed that the majority of the flanking peaks correspond to protein with correct sequence but having an aggregation state different from the major peak.

While a number of different techniques may be used to chemically characterize the hydrogel, a common method used is nuclear magnetic resonance, here the gel is dried and deuterized and then the NMR spectrum is used to determine the composition of the various constituent building blocks that are used to prepare the gel. [75, 80] Sometimes the molecular weight distribution of the monomers is also determined using size exclusion chromatography [80]. 

Samples of bisphenol Apolycarbonate were characterised by DSC, TGA, FTIR spectroscopy, size exclusion chromatography(SEC), vapour pressure osmometry (VPO) and viscometry. The samples exhibited the same FTIR spectrum profile and almost the same values of Tg and initial temp, of degradation. Slight differences were, however, observed in differential TGA curves, which could be due to the presence of additives. The number-... 

Fig. 38 MALDI mass spectrum of poly(PDBF) synthesized using (-)-Sp-FlLi (THF-soluble, MeOH-insoluble part, Mn 5,950 as determined by size-exclusion chromatography) [matrix, 2,5-dihydroxybenzoic acid]. Reprinted with permission from Nakano et al. [34]. Copyright 2004, The Royal Society of Chemistry...

The presence of three repeating units in terpolymer PI, in the same proportion as in the monomer feed, has been demonstrated by NMR spectrum. DSC analysis has given only one glass transition temperature (Tg) at 17 °C. Molecular weight (Mw) and polydispersity index (Ip) were determined by size exclusion chromatography (Mw = 6000, Ip = 1.4 SEC in THF, standards polystyrene). 

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