Size-Exclusion Chromatography of Peptides and Proteins

the monophosphates elute, then the diphosphates follow much later, because of the double charge. 

This broadens the field of application even further. 

SEC is a special form of chromatography, which differs in the fact that there are (ideally) no interactions with the stationary phase. 

Separation is, therefore, based on the different available volumes. In the following, the volume types for a column of 300 x 8 mm are specified. 

Volume of the mobile phase (dead volume) Vq Volume of the stationary phase 

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