Size exclusion chromatography Light scattering techniques

P.J. Wyatt, Light scattering and the solution properties of macromolecules, in Handbook of Size Exclusion Chromatography and Related Techniques, vol. 91 (Chromatographic Sciences Series), C.-s. Wu, ed., Marcel Dekker, Inc., New York, 2004, p. 623 Anal. Chim. Acta 111, 1, 1993. 

Beri, R. G., Walker, J., Reese, E. T., and Rollings, J. E., Characterization of chitosans via coupled size-exclusion chromatography and multiple-angle laser light-scattering techniques, Carb. Res., 238, 11, 1993. 

We report here the results of our recent studies of poly(alkyl/arylphosphazenes) with particular emphasis on the following areas (1) the overall scope of, and recent improvements in, the condensation polymerization method (2) the characterization of a representative series of these polymers by dilute solution techniques (viscosity, membrane osmometry, light scattering, and size exclusion chromatography), thermal analysis (TGA and DSC), NMR spectroscopy, and X-ray diffraction (3) the preparation and preliminary thermolysis reactions of new, functionalized phosphoranimine monomers and (4) the mechanism of the polymerization reaction. 

The experimental techniques for the study of conformational branched properties in solution are the same as used for linear chains. These are, in particular, static and dynamic light scattering, small angle X-ray (SAXS) and small angle neutron (SANS) scattering methods, and common capillary viscometry. These methods are supported by osmotic pressure measurements and, nowadays extensively applied, size exclusion chromatography (SEC) in on-line combination with several detectors. These measurements result in a list of molecular parameters which are given in Table 1. 

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