Size exclusion chromatography isolation

In addition to the insoluble polymers described above, soluble polymers, such as non-cross-linked PS and PEG have proven useful for synthetic applications. However, since synthesis on soluble supports is more difficult to automate, these polymers are not used as extensively as insoluble beads. Soluble polymers offer most of the advantages of both homogeneous-phase chemistry (lack of diffusion phenomena and easy monitoring) and solid-phase techniques (use of excess reagents and ease of isolation and purification of products). Separation of the functionalized matrix is achieved by either precipitation (solvent or heat), membrane filtration, or size-exclusion chromatography [98,99]. 

Analysis of the MHR by high-performance size-exclusion chromatography (HPSEC) revealed three distinct populations to be present, which were isolated on a preparative scale. The composition of the populations differed mainly in the relative proportion of rhamnose, xylose and the amount of methyl esters and acetyl groups, although the general characteristics were rather similar (Table 2). However, degradation studies with RGase showed that, in contrast... 

Sugar beet pulp was saponified and then hydrolysed with 0.1 N HCl at 80 °C for 72 h. Linear RG oligomers of DP 6 to 18, abbreviated RGO 6 to 18, were isolated by ion-exchange chromatography and size-exclusion chromatography by Renard et al. (1995). 

Saponified apple MHR was treated with RG-hydrolase, and the branched RG-hydrolase MHR oligomers were isolated using size-exclusion chromatography as described in Mutter et al. (1994). A similar procedure was carried out with RG-lyase, and the branched RG-lyase MHR oligomers were isolated as described in Mutter et al. (1996). 

The monodispersed nature of dendrimers has been verified extensively by mass spectroscopy, size exclusion chromatography, gel electrophoresis and electron microscopy (TEM). As is always the case, the level of monodispersity is determined by the skill of the synthetic chemist, as well as the isolation/purification methods utilized. 

The ELISA can be used as one component of a battery of analyses. Rarely is only one method used in isolation. Other tests include chromatographic methods such as reversed-phase high-performance liquid chromatography (HPLC), size exclusion chromatography, and physical structure analytical methods such as UV spectral analysis, mass spectroscopy, etc. 

Size exclusion chromatography also known as gel filtration has been extensively used as a part of the isolation protocol of various biological molecules. This mode of application will not be considered, instead this article will concentrate on examples that use SEC as the only fractionation technique in combination with detection methods that help to give an indication of the type of molecules associated with the metal. 

Recovery, Purity and Amino Acid Composition of CMP. The elution profiles of the CMP powders obtained on size exclusion chromatography 23) are shown in Figure 6. Both CMP isolated from WPC and whey did not contain major... 

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