Size exclusion chromatograph molecular mass detector

The bottom line conclusion of this Section can be stated thus If two analytes A and B are to be chromato-graphically separated, their adjusted retention volumes V, and V,g (Equation [3.15]), and thus the corresponding retention times tj, must be sufficiently different. To separate two solutes, either their distribution coefficients and K (Equation [3.1]) must be made to differ (choose appropriate phase systems) or the volumes of stationary phase with which they interact must be made to differ (choose a stationary phase with appropriate exclusion properties based on molecular size so that the effective values of are different for different analytes) or a shrewd combination of both. It is appropriate to mention that if mass spectrometric detection is used, clean chromatographic separation of solutes in the analytical extract is not as crucial as for less selective detectors in view of the additional selectivity provided by the m/z information. 

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