SiRNA-polyelectrolyte complexes

FIGURE 2.1.8 (a) Design of virus-like polymer particles by self-assembling amphiphilic block copolymer molecules around RNA-based polyelectrolyte complexes, (b) Uptake and intracellular distribution of bilayer siRNA-polyethylenimine (PEI) complexes prepared at different concentrations of copolymer into HeLa cells after 24 h of incubation ([siRNA] = 50 nM). Copolymer and siRNA molecules are labeled with fluorescein and Cy5, respectively. Last column Overlay of DIC (differential interference contrast) and fluorescence images (blue nuclei stained with DAPI [4, 6-diamidino-2-phenylindoleD [108]. 

S. H. Lee, S. H. Kim and T. G. Park, Intracellular siRNA delivery system using polyelectrolyte complex micelles prepared from VEGF siRNA-PEG conjugate and cationic fusogenic peptide. 

---