Single-peak correlation chromatography

RCs were isolated from Rb. sphaeroides w.t. by anion exchange chromatography. The measurements were carried out by the time-correlated single-photon-counting technique as described. The full width at half maximum of the system temporal response function was about 30 ps. All measurements were carried out at room temperature (22°C) accumulating up to about 20000 counts in the peak channel with a time resolution per channel of 2.0 ps. From test measurements we verified that we are able to easily resolve lifetimes down to about 2 to 3 ps. The sample was circulated through a flow cell from a dark reservoir in order to keep the RCs in the open state. Excitation occurred at a wavelength of 800 nm. The AG-values for the various reaction steps are also calculated based on the relationship between the forward and reverse rate constants ... 

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