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Single molecule fluorescence experiments sample presentation

Even with the most careful protocols it may be impossible to remove all contaminants from a solution that produce a signal which maybe mistaken for valid single molecule events. Of particular concern are small fluorescent molecules, free fluorescent dye and particulates that can scatter large amounts of the excitation light. Control experiments involving the solutions without the fluorescence molecules of interest should always be carried out in order to minimize and fully characterize the background contribution. Further details on sample preparation protocols that have been found to be satisfactory are presented in Chapter 4. [Pg.64]


See other pages where Single molecule fluorescence experiments sample presentation is mentioned: [Pg.4]    [Pg.100]    [Pg.161]    [Pg.271]    [Pg.480]    [Pg.266]    [Pg.76]    [Pg.193]    [Pg.200]    [Pg.216]    [Pg.3123]    [Pg.121]    [Pg.231]    [Pg.593]    [Pg.2410]   
See also in sourсe #XX -- [ Pg.100 ]




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