Simultaneous substrates utilization in single cell

Besides S. cerevisiae, Zymomonas mobilis and C. acetobutylicum have been also engineered with metabolic pathway for xylose and/or arabinose utilization. Clostridium 

The introduction of a catabolic pathway for the second compound would expand the substrate spectrum of the host. It could also significantly increase the synergy between the different substrates, which could increase the productivity of the desired products, improving the fermentation economics. 

For example, in order to produce polyhydroxybutyrate (PHB) from a mixture of xylose and glucose, Lee et al. knocked out the ptsG gene, which encoded a glucose-specific enzyme, in phosphoenolpyruvate-dependent glucose-specific PTS 

3 Global Analysis and Modification of the Substrates Coutilization System 

---

SIMULTANEOUS SUBSTRATES UTILIZATION IN SINGLE CELL 2.1 Introduction of Heterologous Substrate Catabolic Pathway... 

Simultaneous substrate utilization is the prerequisite for single-cell biorefinery. The selected microbes must possess the possibility to utilize the multiple compounds in the substrate mixture or biomass hydrolysates. However, to design a single-cell biorefinery system, the coproduction in single cell is necessary. 

---