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Simultaneous Immunoenzymatic Double Staining

A collection of useful protocols for the simultaneous immunoenzyme staining of two or more tissue antigens is available on the websites http //www.protocol-online.org/prot/Immunology/ http //www.vectorlabs.com/Protocols/MLB.pdf and http //www.ihcworld.com/. The protocols given in this chapter are practiced in the authors laboratory. These methods fall into two main categories (a) simultaneous immunoenzymatic double staining, and (b) sequential immunoenzymatic double/ multiple staining. [Pg.61]

Buchwalow and W. Bocker, Immunohistochemistry Basics and Methods, DOI 10.1007/978 3 642 04609 4 7, Springer Verlag Berlin Heidelberg 2010 [Pg.61]

Basic protocol for simultaneous immunoenzymatic double staining using primary antibodies of two different species or two different IgG isotypes [Pg.62]

Deparaffinize and rehydrate tissue sections. Rinse in distilled water for 5 min. [Pg.62]

Antigen retrieval place sections in a Coplin jar with antigen retrieval solution of choice (e.g., 10 mM citrate acid, pH 6) and heat at 90 110°C (depending on tissue) in a microwave, steamer, domestic pressure cooker or autoclave (see Sect. 6.1.1). [Pg.62]


See other pages where Simultaneous Immunoenzymatic Double Staining is mentioned: [Pg.61]    [Pg.61]    [Pg.62]    [Pg.65]    [Pg.61]    [Pg.61]    [Pg.62]    [Pg.65]    [Pg.451]   


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