Silver staining plasma proteins

Using the more sensitive silver stain, however, Goldman and coworkers (G9) have been able to resolve more than 300 proteins and/or peptides (denatured sample) in csf using lEF/SDS-PAGE (Fig. 14). Twenty six of the proteins have been tentatively identified (Fig. 15), and comparison of the protein patterns with the corresponding plasma protein patterns has revealed several clusters of unidentified proteins that were more prominent in csf than in plasma and hence, may be of CNS origin. 

Protein Adsorption. Since protein adsorption is the earliest event in the thrombotic response of the blood to polymers, the types of proteins adsorbed from plasma to polyacrylamide-Silastic and Silastic tubes were examined. As described in the Experimental section, plasma was recirculated through the tubes for 2 h at 37°C and rinsed away with buffer. The proteins adsorbed to the polymers were eluted with SDS, separated by SDS-polyacrylamide slab gel electrophoresis, and silver-stained using a new extremely sensitive method (19). Figure 10 shows the results of these assays. 

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