Silver Staining of Proteins in Gels

The following procedures are well improved and easy to perform. Nevertheless, in some cases variations of the protocols concerning silver binding and/or reduction can give better or other results, because proteins bind silver differently and are observed with varying colors. Some hints are given in the cited papers. 

The solution is prepared freshly before using. After use, the solution has to be destroyed by hydrochloric acid. 

E reduction solution 0.025 g citric acid and 0.185 ml 27% formaldehyde in 100 ml ddH2 0 (stabilize for a longer period if frozen) 

The gel to be stained should not be thicker than 1 mM for that diffusion of the reactants is quickly. The volumes of used solutions should be at least the tenfold of the gel volume. 

The gel is moved gently during all steps. Especially immediately before, during, and after the application of Soln. D the gel must not be touched with unprotected fingers (use gloves and/or tweezers) or come in contact with chloride-containing solutions. 

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