Silver enhancement process

Incubate tissue sections in the NPG silver enhancement solution under a sodium vapor safelight until labeling of test strip is similar to that determined previously as acceptable. To be sure of getting the ideal silver enhancement time, it is best to use two or three times for samples to process for the rest of the procedure. 

Figure 2 Mouse small intestine processed by PLT into Lowicryl HM20. The rough ER is labelled with an anti-PDI (protein disulfide isomerase). (a) Low power view the 5 nm gold conjugate was silver enhanced for 12 min. Magnification x 21000. (b) Higher power view where the 5 nm gold was enhanced for 6 min. Magnification x 62000.

Replacement of silver nitrite by inexpensive sodiiunor potassium nitrite enhances the imlity of this process Treatment of alkenes v/ith sodiiun nitrite and iodine in ethyl acetate and water in the presence of ethylene glycol gives conjngatednitroalkenesin49-82% yield The method for generation of nitryl iodide is improved by the treatment of iodme v/ith potassium nitrite complexed v/ith 18-crovm-6 in THF under sonicadon, as shovmin Eq 2 32 ... 

This study relates to a continuous process for the preparation of perfluoroalkyl iodides over nanosized metal catalysts in gas phase. The water-alcohol method provided more dispersed catalysts than the impregnation method. The Cu particles of about 20 nm showed enhanced stability and higher activity than the particles larger than 40 nm. This was correlated with the distribution of copper particle sizes shown by XRD and TEM. Compared with silver and zinc, copper is better active and stable metal. 

The surface of the silver reaction channels was enhanced by means of the oxidation and outgassing reduction (OAOR) process, which relies on oxidation at 250 °C using pure oxygen and subsequent reduction. An increase in surface area by a factor of 2-3 was reached as indicated by chemisorption data. 

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