Silica antibody-conjugated

These Ru(H)bpy32+ fluorescent silica nanoparticles were used to detect single bacterial cells using antibodies conjugated to the surface after functionalization with trimethoxysilyl-propyldiethylenetriamine followed by succinylation to create carboxylates. Specific antibody molecules against E. coli 0157 then were coupled to this modified fluorescent particle using the carbodiimide method with EDC and NHS (Zhao et al., 2004). 

Figure 11.4 Schematic representation of the multiple extraction procedure with the antibody-conjugated silica nanoparticles doped with magnetic nanoparticles (MNP) being added and extracted stepwise and the corresponding antibody-conjugated silica nanoparticles doped with fluorescent dyes (FNP) being added post-magnetic extraction of cell samples.40 (Reprinted with permission from J. E. Smith et al., Anal. Chem., 2007, 79, 3075-3082. Copyright 2007 American Chemical Society.) (See color insert.)...

Figure 11.5 Scheme of sandwich fluoioimmunoassay for the determination of human IgG (immunoglobulin G) with goat anti-human IgG antibody-conjugated silica nanoparticles (Ab-NH2-AlC4Pc-Si02 NPs)41... 

Cai, W. (2013) In vivo tumor targeting and image-guided drug delivery with antibody-conjugated, radiolabeled mesoporous silica nanoparticles. 

TLC/immunostaining has been used to detect solaso-dine glycosides by separation of the compounds on a silica gel layer, transfer to a polyvinylidene difluoride membrane, and treatment of the membrane with sodium periodate solution followed by bovine serum albumin (BSA), resulting in a solasodine-BSA conjugate. Individual zones were stained by monoclonal antibody against solamargine. 

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