Silanisation silica, reaction

Levarato has developed a series of quantitative methods for the determination of prednisolone along other corticoids after extraction from preparations and separation by TLC on silica gel plates and determination by spectrophotometric (absorption at 240 nm) or colorimetric methods (INH-hydrazone formation, tetrazolium reaction) (81). A simple, fast and quantitative TLC-method for the determination of prednisolone in tablets is described. The method is stability-indicating with respect to accuracy, specificity, sensitivity and precision. The coefficient of variation was between 1.26 and 1.96 % and the sensitivity was about 25 ng. The chromatographic separation was performed on a silicagel plate using two step development of the plate (82). A simple TLC method of separation of prednisolone from other corticosteroids is reported (83). Prednisolone and the dephosphorylated D-homosteroid can be separated on silanised silica gel TLC plates (36). 

Effect of Rubber Type on the Silica Silanisation Reaction... 

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