Sialic acid-imprinted polymer

Fig. 20.7. Compounds examined using sialic acid-imprinted polymer.

With regard to substrate-selective sensors with pre-organized cavities, impressive advances have been made in molecular imprinting [55-57]. The discovery of MIP-membrane electro conductivity was an interesting issue, which actually led to the appearance of the earliest MIP sensors [58,59]. It was shown that the membrane electroconductivity could be a function of the interaction between MIP-membrane and ligand (i.e., imprint species) (Fig. 5). An increase in the ligand concentration would result in an enhancement of membrane conductivity. With the same level of concentration, a maximal electro conductivity with the imprint species could be achieved. In addition, it has also been confirmed that polymers imprinted with amino acids, nucleosides, atrazines, sialic acids, or cholesterols can show similar features if coupled with the appropriate transducer [60-64], In particular, molecular imprinting is presently probably the only choice when no suit-... 

A surface plasmon resonance (SPR) sensor using a molecularly imprinted polymer-coated sensor chip for the detection of sialic acid was reported [19]. The thinly coated polymer was prepared by co-polymeriz-ing N, N, N-trimethylaminoethyl methacrylate, HEM A, and EDMA in the presence of the p-vinylbenzeneboronic acid ester of sialic acid. The sensor selectively responded to a ganglioside of which sialic acid is located at the non-reducing end and gave a linear relationship from 0.1 to 1.0 mg of the ganglioside. 

Kugimiya, A. Takeuchi, T. Surface plasmon resonance sensor using molecularly imprinted polymer for detection of sialic acid. Biosens. Bioelectron. 2001,16,1059-1062. 

A. Kugimiya, J. Matsui, T. Takeuchi, K. Yano, H. Muguruma, A. V. Elgersma, et al, Recognition of Sialic-Acid Using Molecularly Imprinted Polymer, A/raZ. Lett., 1995,28(13), 2317-2323. 

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