Shah convolution Fourier transform detection

Multiple-point fluorescent deteclion has been proposed to enhance detection sensitivity. This method is based on the use of a detector function, such as the Shah function. The time-domain signals were first detected, and they were converted into a frequency-domain plot by Fourier transformation. Therefore, this technique was dubbed Shah convolution Fourier transform detection (SCOFT). As a comparison, the single-detection point time-domain response is commonly known as the electropherogram [698,699,701]. 

Crabtree, H.J., Kopp, M.U., Manz, A., Shah convolution Fourier transform detection. Anal. Chem. 1999, 71, 2130-2138. 

McReynolds, J.A., Edirisinghe, P., Shippy, S.A., Shah and sine convolution Fourier transform detection for microchannel electrophoresis with a charge coupled device. Anal. Chem. 2002, 74, 5063-5070. 

Among the mathematical transformation techniques, CC and HT substantially improve the S/N ratio in CE separations. It should be noted that Shah convolution Fourier transform (SCOFT) detection yielded results that differ from those obtained with either CC or HT. Although fundamental studies have demonstrated the potential of SCOFT (6,11), both the advantages and disadvantages remain to be determined. Nevertheless, SCOFT apparently has the potential to improve the resolution and/or sensitivity of microchip electrophoresis (ME). In this chapter, we discuss the principles, instrumentation, and performance of CE and ME methods using CC, HT, and FT techniques. 

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