Serum-resistant lipoplex

Add 1 ml transfection medium with the lipoplex to each well, ensuring that serum is not present at this step (adjust for different well sizes). Some lipids are maybe serum resistant which allows for use with serum containing media. 

Faneca H, Simoes S, Pedroso de Lima MC. Association of albumin or protamine to lipoplexes enhancement of transfection and resistance to serum. J Gene Med 2004 6 681. 

Fig. 6 Basic characterization and transfection activities of PEG-PLL/pDNA micelle, a Size distribution of the PIC micelle (PEG-PLL (12-48)/pDNA, charge ratio 1.0) obtained from histogram analysis of dynamic light scattering (DLS). b Nuclease resistance as a function of PLL length evaluated by an increase in absorbance at 260 nm by the addition of DNase I in 10 mM tris-HCL buffer, pH 7.4 (i) Native DNA+ 11 U DNase I, (ii) PEG-PLL12-7/DNA complex + 110 U DNase I, (iii) PEG-PLL12-19/DNA complex+ 110 U DNase I, (iv) PEG-PLL12-42/DNA complex + 110 U DNase I), c Charge ratio dependency of transfection activity of PIC micelles with varying compositions (12-7, 12-19, 12-48) against 293 cells (n = 4, S.D. + 100 p.M HCQ). d Influence of preincubation with serum on transfection activity of PIC micelles (12-48, r = 2), polyplex (PLL/pDNA, r = 2), and lipoplex system (LipofectAMINE/pDNA complex). The preincubation of these complexes in 20% serum was done for 30 min prior to transfection ( = 4 SD). (Fig. 6a,c,d Reprinted with permission from [87] and Fig. 6b from [81])...

Simoes, S., Slepushkin, V., Fires, R, Caspar, R., Pedroso, D. L. M., Duzgunes, N. 2000. Human serum albumin enhances DNA transfection by lipoplexes and confers resistance to inhibition by serum. Biochim. Biophys. Acta 1463 459 69. 

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