Serum glycoproteins fetuin

Figure 11.25 Mass spectrometric sequencing of oligosaccharides. Carbohydrate-cleaving enzymes were used to release and specifically cleave the oligosaccharide component of the glycoprotein fetuin from bovine serum. Parts A and B show the masses obtained with MALDI-TOF spectrometry as well as the corresponding structures of the oligosaccharide-digestion products (using the same scheme as that in Figure 11.18) ...

Carbohydrate-cleaving enzymes were used to release and specifically cleave the oligosaccharide component of the glycoprotein fetuin from bovine serum. 

Oshiro, Y., and Eylar, E. H., 1968, Physical and chemical studies on glycoproteins. III. The microheterogeneity of fetuin, a fetal calf serum glycoprotein. Arch. Biochem. Biophys. 127 476-489. 

Neeser and Schweizer introduced 4 M CF3CO2H for 1 h at 121° for hydrolysis of glycoproteins. Both neutral and amino sugars were considered. They compared this method to hydrolysis with 0.6 M hydrochloric acid for 4 h at 100° and 3 M hydrochloric acid for 0.75 h at 125°. Hydrolysis of fetal-calf-serum fetuin, bovine submaxillary mucin, and horse-radish peroxidase showed hydrolysis with CF3CO2H to be superior. 

The strategy used to perform the PMP oligosaccharide profile analysis, with the use of bovine fetuin as a model system, is given in Scheme 1. Bovine fetuin, a major glycoprotein in fetal calf serum, has been widely used as a model for the study of glycoprotein structure. This glycoprotein contains both N- and O-linked oligosaccharides and detailed structural information is available (9-11). 

Recently, a new polyacrylamide (PAA) derivative that allows dynamic coating (UltraTrol LN- Target Discovery, Palo Alto, CA) has been reported for the efficient separation of glycoforms of ATT [70], A comparison of the ability of PB and UltraTrol LN for the separation of bovine alpha-1-acid glycoprotein (AGP), bovine serum fetuin (BSF), and rhEPO sialoforms and their application coupling with MS has been reported [30,71]. Better resolution of sialoforms was obtained with capillaries coated with UltraTrol-LN in acidic volatile buffers used for coupling CE to ESI-TOF MS. 

Bovine Fetuin. See Glycoproteins Bovine Serum Albumin Sol n.. See Serum albumin... 

The serum iGnT transferred GlcNAc effectively also to desialylated fetuin and desialylated ai-acid glycoprotein and to the pentasaccharides Gal(pi-4)-GlcNAc(pi -2)Man(al -3)Man(p 1 -4)GlcNAc and Gal(pl -4)GlcNAc(pi-2)-... 

Spiro, R. G., 1960, Studies on fetuin, a glycoprotein of fetal serum I. Isolation, chemical composition, and physicochemical properties, J. Biol. Chem. 235 2860-2869. 

---