Serum bottles

The plot of the rate of disappearance of CO per volume of liquid in the serum bottles versus partial pressure of CO in the gas phase based on (3.14.4.14) could give the constant slope value of KLa/H. Henry s constant is independent of the acetate concentration but it is only dependent on temperature. The overall volumetric mass transfer coefficient can be calculated based on the above assumption. The data for various acetate concentrations and different parameters were plotted to calculate the mass transfer coefficient. 

Miller TL, MJ Wolin (1974) A serum bottle modification of the Hungate technique for cultivating obligate anaerobes. Appl Microbiol 27 985-987. 

First, the capacity of the fungus T. versicolor to degrade deca-BDE was evaluated. To carry out the treatment, the fungus was used in the form of pellets 100 mL amber serum bottles were filled with 10 mL of defined medium (DM) with Tween 80 (500 mg L x) as surfactant to increase the solubility of the contaminant and deca-BDE at a concentration of 10 mg L 1. The bottles were inoculated with the necessary amount of pellets to reach an approximate biomass concentration of 3.5 g L-1 (dry weight). 

L-glutamic acid and 30 mM malic acid under the same experimental condition as for the pre-culture. The 150 ml-capacity of a serum bottle was used for the pre-cultures and for the hydrogen production. Two types of 2.5 L-capacity flat-rectangular and vertical photo-bioreactors, which were made of glass for both flat sides and stainless steel for the edges or made of clear plastic acryl material, were used for the production of hydrogen and P-D-hydroxybutyrate. 

Cells were grown in the 50 ml serum bottles using the modified Sistrom s broth at 30°C for 48 hr under 8 Klux/m2 irradiance. 

The effect of mineral and organic soil constituents on the mineralisation of LAS, AE, stearyl trimethylammonium chloride (STAC) and sodium stearate (main soap component) in soils was studied by Knaebel and co-workers [38]. The four 14C-labelled compounds were aseptically adsorbed to montmorillonite, kaolinite, illite, sand and humic acids and subsequently mixed with soil yielding surfactant concentrations of about 50 jig kg-1. The CO2 formation in the serum bottle respirometers was monitored over a period of 2 months indicating that the mineralisation extent was highest for LAS (49-75%). Somewhat lower amounts of produced CO2 were reported for AE and the stearate ranging from 34-58% and 29-47%, respectively. The mineralisation extent of the cationic surfactant did not exceed 21% (kaolinite) and achieved only 7% in the montmorillonite-modified soil. Associating the mineral type with the mineralisation kinetics showed that sand... 

Results from the two experimental studies of Cr isotope fractionation are summarized in Table 6. An initial set of experiments has proven that Cr(VI) reduction induces a kinetic isotope effect. Ellis et al. (2002) reduced Cr(VI) to Cr(III) in anaerobic serum bottles using... 

Strain K4 was tested in the absence of oxygen at various concentrations of glycerol (10,20,30,40 g L ).Two serum bottles were used with a modified Hungate technique one ofthe bottles contained resazurin and a reducing agent, namely cysteine-H Cl, and the other did not contain resazurin. The gas was analyzed by GC with TCD. 

Battersby NS, Wilson V. 1988. Evaluation of a serum bottle technique for assessing the anaerobic biodegradability of organic chemicals under methanogenic conditions. Chemosphere 17 2441-2460. 

With minimum exposure to the atmosphere, pipet 25 ml of the dry extraction solvent into a 30 ml serum bottle. 

Aquifer- sediment (constitutive degrader of TCE) 100 ml serum bottles 25 flM TCE within 24 h at high cell density, but not at all at lower densities. Sediment microcosms high densities removed all TCE overnight, lower densities took much longer (weeks). (1993)... 

Volatile reactive liquids present few problems since they generally can be distilled from the vacuum line into a tube equipped with a serum bottle cap (Fig. 9.26). Nitrogen is then admitted to the tube, and the sample is taken with a syringe. Another scheme involves an inlet with a capillary tube or ampule breaker.33 35 This method is potentially useful for vacuum line work, since it is relatively simple to fill and seal off a sample tube attached to the vacuum line. 

All chemicals were laboratory grade and dissolved in distilled water. The medium was sterilized by autoclaving, and sorption experiments were performed in sealed 150-mL serum bottles. Subsequent tests for in situ use may need to be performed at mesophilic fermentation temperatures. In a similar manner, the sorbents were also tested for their capacity for the substrate of the possible fermentation, glucose, at a concentration of about 5 g/L. Frequently the glucose was included in the fermentation medium for competitive sorption. 

Sample Preparation Prepare four 50-mL serum bottles. Pipet 2.0 mL of Internal Standard Solution into each bottle. Pipet 5.0, 10.0, or 20.0 iL of the Standard Addition Solution, equivalent to about 21.6, 43.2, and 86.3 pig of methanol, into three of the bottles, and evaporate the solvent at room... 

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