Sequential analysis monosaccharide sequences

A number of glycosidic hydrolases have been produced in sufficiently pure form to allow the development of a method of determination of monosaccharide sequences based on these enzymes (Table 6.4). These enzymes will remove specific monosaccharide units linked by specific linkages from the nonreducing end of a polysaccharide. For example, P-galactosidase will remove D-galactosyl residues linking p-glycosidically to polysaccharide. The use of sequential enzymatic hydrolysis is a well-established technique, particularly for the analysis of the carbohydrate residues of macromolecules. 

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