Sequencing, of peptides

Besides such textual databases that provide bibhographic information, sequence databases have attained an even more important role in biochemistry. Sequence databases are composed of amino add sequences of peptides or proteins as well as nudeotide sequences of nudeic acids. The 20 amino adds are mostly represented by a three-letter code or by one letter according to the biochemical conventions) the four nudeic adds are defined by a one-letter code. Thus the composition of a biochemical compound is searchable by text retrieval methods. 

Edman degradation (Section 26.6) A method for N-terminal sequencing of peptide chains by treatment with Af-phenylisothiocyanate. 

The primary structure - the sequence of peptide-bonded amino acids in the protein chain and the location of any disulfide bridges. 

Fig. 16 (a) Sequences of peptides pi6 and B2x-pi6. (b) Biotin-derivatised colibrils binding to a functionalised streptavidin with a functional group. Reproduced by permission of The Royal Society of Qiemistry from Kodama et al. [56]... 

Figure 5. Partial peptidic sequences of peptides resulting fi-om tryptic cleavage PME isoforms.

Faller T, Hutton K, Okafo G, et al (1997) A novel acridone derivative for the fluorescence tagging and mass spectrometric sequencing of peptides. Chem Commun 1529-1530... 

PITC has been used extensively in the sequencing of peptides and proteins and reactions under alkaline conditions with both primary and secondary amino acids. The methods of sample preparation and derivatization follow a stringent procedure which involves many labour-intensive stages. However, the resulting phenylthio-carbamyl-amino acids (PTC-AA s) are very stable, and the timing of the derivatization step is not as critical as when using OPA. 

D. De novo Sequencing of Peptides with Mass Spectrometry. 15... 

Naven, T., Canas, B., Rahman, D., Bardetjones, M., Jeffrey, W., and Pappin, D. (1999). De novo sequencing of peptides by low-energy CID. 47th ASMS Conference, June 13-17, Dallas. 

Table 2.2 Molecular masses and amino acid sequences of peptides isolated from the extract of acrospires of Arena sativa oat...

Kaufmann, R. Kirsch, D. Spengler, B. Sequencing of Peptides in a TOF Mass Spectrometer Evaluation of Postsource Decay Following MALDL Int. J. Mass Spectrom. lonProc. 1994, 757,355-385. 

Because of their known specificity, these enzymes, especially trypsin and chymotrypsin, have been widely utilized in helping to determine the amino acid sequences of peptides (see Section 13.7). Hydrolysis using these... 

Figure 14.8 (Left) Primary sequence of peptide MAXI with /8-hairpin promoted intramolecular folding, leading to the reversible formation of self-assembled /8-sheets. (Right) Cryo-TEM image of self-assembled peptide scaffolds. Scale bar = 200 nm. Reprinted from Schneider et al. (2002). Copyright 2002 American Chemical Society.

Figure 14.8 (Left) Primary sequence of peptide MAXI with /3-hairpin promoted intramolecular folding, leading to the reversible formation of self-assembled...

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