Sequencing of oligosaccharides

P.M. Rudd, R.A. Dwek, Rapid, sensitive sequencing of oligosaccharides from glycoproteins, Curr. Opinion Biotechnol., 8 (1997) 488. 

Figure 11.25 Mass spectrometric sequencing of oligosaccharides. Carbohydrate-cleaving enzymes were used to release and specifically cleave the oligosaccharide component of the glycoprotein fetuin from bovine serum. Parts A and B show the masses obtained with MALDI-TOF spectrometry as well as the corresponding structures of the oligosaccharide-digestion products (using the same scheme as that in Figure 11.18) ...

The microheterogeniety, i.e. consequence of multiple glycosylation sites and partial site-occupancy of a population of different oligosaccharide structures, results in a set of glycosylated variants of a common polypeptide. These glycoforms differ in the nature, number, location and sequence of oligosaccharides, which affect their properties and... 

Edge, C., et al. (1992a). Fast Sequencing of Oligosaccharides The Reagent-array Analysis Method, Proc. Natl. Acad. Sci. USA 89 6338-6342. 

S. Hanrahan, J. Charlwood, R. Tyldesley, J. Langridge, R. BordoU, R. Batman, and P. Camilleri, Facile sequencing of oligosaccharides by matrix-assisted laser desorption/ ionisation on a hybrid quadrupole orthogonal acceleration time-of-flight mass spectrometer, Rapid Commun. Mass Spectrom. 15, 1141-1151 (2001). 

Morelle, W. MlCHALsm, J.-C. Sequencing of oligosaccharides derivatized with benzylamine using electrospray ionisation-quadrupole time of flight-tandem mass spectrometry. Electrophoresis 2004, 25, 2144-2155. 

A total of 27 oligosaccharides composed of hexopyranose, deoxyhexopyranose, deoxyfluoro hexopyranose and 2-acetamido-2-deoxy hexopyranose units have been studied by FAB-m.s.l Structurally significant ions were observed, and the authors suggest that this method will be important for the sequencing of oligosaccharides without prior recourse to deiivatization. 

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