Separator Acclaim HILIC

Acclaim HILIC-10 is similar to Acclaim Mixed-Mode WAX-1. This relatively new column is also based on high-purity spherical silica covalently modified with a proprietary hydrophilic layer. It is suited for use in a broad range of applications including the separation of hydrophilic drugs and their metabolites. Acclaim Mixed-Mode HILIC-1 is a stationary phase that combines both RP and HILIC properties. Unlike traditional RP or HILIC columns, this packing material features a long alkyl chain with a diol functionality at the terminal end. 

Figure 7.21 shows a typical separation of hydrophilic pharmaceutical drugs and some degradation compounds such as salicylic acid on Acclaim HILIC-10 using UV detection at 230 nm. With a mobile phase consisting of acetonitrile and ammonium acetate at pH 5, all five components can be separated to baseline within a reasonable time. Penicillin G is an antibiotic that belongs to the -lactam fomily it is often formulated in the potassium salt form. Because both the API... 

Figure 7.21 Separation of hydrophilic pharma- acetate, pH 5 flow rate 1 mUmin detection ceuticals on Acclaim HILIC-10 under isocratic UV (230nm) injection volume 2 pU sample conditions. Column dimensions 150 mm x4.6 0.1 mg/mt of acetaminophen (1) and salicylic...

Figure 10.256 HILIC separation of meiamine and cyanuric acid with charged aerosoi detection. Separator column Acclaim HILIC-10,...

Figure 7.8 Comparison of the chromatographic behavior between Acclaim Mixed-Mode HILIC-1 and LiChrosorb Diol. Separator columns Acclaim Mixed-Mode HILIC-1,5 pm ( ) and LiChrosorb Diol, 5 pm ( ) column...

Figure 7.9 Comparison of the chromatographic behavior of pharmaceuticai counterions between Acclaim Trinity PI (a) and ZIC-HILIC (b). Separator columns lOOmmxSmm i.d. Acclaim Trinity PI, 3 pm, and 150mmx4.6 mm i.d. ZIC-HILIC, 5 pm coiumn temperature 30 °C eiuent (A) 60 40 (v/v) MeCN/0.02 moi/L NH4OAC, pH 5 and eiuent (B) 80 20 (v/v) MeCN/0.015 moi/L NH4OAC, pH 5 flow rates ...

Figure 7.11 Comparison of the chromatographic behavior of a hydrophobic acidic API and its counterion between Acclaim Trinity PI (a) and ZIC-HILIC (b). Separator columns ...

Figure 7.16 Separation of selected amino acids under HILIC conditions on Acclaim Mixed-Mode WAX-1. Column dimensions 150mmx4.16mm i.d., 5pm column temperature 30 °C eluent 75 25 (vAr) MeCN/25mmol/L...

Figure 7.22 Separation of penicillin G on Acclaim Trinity PI under RP and HILIC conditions. Column dimensions SOmmxSmm i.d., 3 urn column temperature SOT eluent ...

Figure 7.24 Separation of seiected Good s buffers on Acclaim Trinity PI under HILIC conditions. Column dimensions lOOmmxSmm i.d., 3 pm column temperature 30 °C eluent 85 15 (v/v) MeCN/12 mmol/L ammonium...

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