Separation of wheat proteins

Monomeric albumins and globulins are proteins that have molecular weights in the range of 20,000-30,000 and elute in a small peak at elution times slightly greater than the gliadins. 

The main techniques that can do this are reversed-phase (RP)-HPLC and several electrophoretic methods. The term reversed phase arose when the conventional hydrophilic columns were coated with a hydrocarbon (C8 or C18). Separation by these columns is based on differences in hydrophobicity. Some of the electrophoretic methods useful for studying cereal proteins are discussed in the next section. 

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MG Scanlon, PKW Ng, DE Lawless, W Bushuk. Suitability of reversed-phase high-performance liquid chromatographic separation of wheat proteins for long-term statistical assessment of breadmaking quality. Cereal Chem 67 395-399, 1990. 

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