Separation of Sugars on Buffered Kieselguhr G Layers

Stahl and Kaltenbach [1] observed a marked improvement in resolution of simple sugars on kieselguhr G, weakly buffered with 0.02 M sodium acetate. A mixture of eight simple sugars was resolved in 25 to 30 min with solvent I as shown in Table 198. With acetone as solvent, glucose and arabinose have hi /-values of 50 and 71, respectively [21]. 

Other solvent systems are also satisfactory for the sugars [17]. Acetate-buffered kieselguhr G layers have an optimum capacity of 0.5-2.0 [xg of each sugar [1]. 

Waldi [5] and Kringstad [22] have shown that impregnating kieselguhr G layers with 0.1 M sodium dihydrogen phosphate buffer (pH 5) increases the capacity of the layer to about 25 (xg of each sugar. 

Sodium Sodium Boric acetate phospha acid te, pH 5 

The sugars migrate rapidly in solvent II and a good resolution is obtained in one development [5] (see Table 198). The phosphate buffer in the solvent may be replaced by water with little effect on the hi /-values [5, 22]. 

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