Separation of hydrolysed amino acids

Separation of hydrolysed amino acids The sample is resuspended in 200 p of loading buffer (pH 2.2). An aliquot of 20-100 p is loaded into a loading capsule and the sample injected onto the top of the column. 

A calibration analysis is performed prior to the sample analyses to 

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Figure 3.269 Separation of hydrolysate amino acids and O-phosphorylated amino acids on AminoPac PA10. Eluent NaOH/NaOAc gradient graphically depicted flow rate 0.25 mU min detection see Figure 3.268 chromatogram (a) 200pmol each of arginine (1), hydroxylysine (2), lysine (3), galactosamine (4), glucosamine (5), alanine (6), threonine (7),...

Fig. 3-209. Separation of hydrolysate amino acids and O-phospho lated amino acids on AminoPac PA-10 (250 X 2 mm i. d.). — Eluant NaOH — NaOAc gradient graphically depicted flow rate 0.25 mt/min detection see Fig. 3-206 chromatogram A 200 pmol each of arginine (1), hydroxylysine (2), lysine (3), galactosamine (4), glucosamine (5), alanine (6), threonine (7), glycine (8), valine (9), hydroxyproline (10), serine (11), proline (12), isoleucine (13), leucine (14), methionine (15), norleucine (16), histidine (17), phenylalanine (18), glutamate (19), aspartate (20), cystine (21), and tyrosine (22), chromatogram B 50 pmol each of P-arginine (23), P-serine (24), P-threonine (25), and P-tyrosine (26).

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