Sensors based on separation of the catalyst

Therefore, this sensor integrates a biochemical and a chemical reaction with a prior separation (dialysis) and chemiluminescence detection. The process involves the following steps (a) dialysis of the enzyme (6) enzymatic oxidation of the reagent (c) derivatization of hydrogen peroxide and d) detection of the chemiluminescence produced. Such an original approach offers several advantages over similar methodologies, namely  

The volume of catalyst solution required is only a few microlitres per assay, which is extremely important when using enzyme solutions  

Light emission is localized near the microporous membrane  

The two reactions involved can be sequentially implemented in an expeditious manner at markedly different pH values thanks to the pH gradient established  

The catalyst and analyte are mixed very rapidly under controlled 

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