Semisynthesis of Lipidated LC3 Protein

Autophagy-associated lipidated LC3 protein was prepared using the EPL technique [21]. The choice of the ligation site was considered in order to meet some criteria. First, to perform the EPL under folding conditions, the ligation site should 

The ligation reaction was performed by mixing the lipidated peptide 5 with MBP-LC3 -thioester protein in the presence of MPAA as a catalyst. The 

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